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產(chǎn)品名稱
Branched Chain Amino Acid Assay試劑盒/ BCAA Assay試劑盒
檢測(cè)方法
Colorimetric
樣品類型
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids
檢測(cè)類型
Quantitative
靈敏度
> 0.2 nmol/well
檢測(cè)時(shí)間
0h 40m
產(chǎn)品概述
Branched Chain Amino Acid Assay Kit / BCAA Assay Kit ab83374 provides a simple convenient means of measuring the BCAA's in biological samples.
The BCAA assay protocol uses an enzyme reaction in which BCAA is oxidatively deaminated, producing NADH which reduces a probe, generating a colored product (λmax = 450 nm).
The Branched Chain Amino Acid Assay Kit measures BCAA's in the range of 0 to 10 nmol per sample with a detection limit of ~0.2 nmol (~10 μM BCAA in sample). BCAA's are present in serum ~ 0.1-0.4 mM each (~0.125-1.5 mM combined).
BCAA assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min
- analyze with a microplate reader

研究領(lǐng)域
Signal Transduction 
Metabolism 
Amino Acids
Cell Biology 
Other Antibodies 
Drugs and Chemicals
Kits/ Lysates/ Other 
Kits 
Cell Metabolism Kits 
Amino Acid Assays
相關(guān)性
The branched-chain amino acids or BCAA’s, refer to the amino acids with non-linear aliphatic side-chains, namely leucine, isoleucine and valine. These three essential amino acids make up approximately 1/3 of skeletal muscle in the human body. BCAA’s are currently used clinically to aid in the recovery of burn victims, as well as for strength supplementation for athletes. BCAA’s, primarily Leu, can stimulate insulin secretion. The BCAA’s have also been implicated in a wide range of other physiological effects.
別名
BCAA
Isoleucine
Leucine
Valine